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Immunofluorescent images of formaldehyde-fixed cell lines are shown. Three different organelle markers are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The various cell structures that are demonstrated are always shown in the green channel using an antibody found in the Human Protein Atlas. The antibody id is linked to the corresponding Cell Atlas protein page. By using the "toggle channels"-buttons, the different channels can be turned on and off. Most cell structures can be highlighted in the cell illustration by hovering over them with the exception of the aggresome. Cytoplasmic bodies are highlighted as substructures of cytosol; cytokinetic bridge, midbody, midbody ring and mitotic spindle are highlighted as microtubules, cell junctions are highlighted as plasma membrane and nucleus is highlighted as nucleoplasm.
The cell structure section of the dictionary is based on representative images of antibody-stained human cancer cell lines. Each sample is stained with an in-house generated HPA-antibody and counterstained with markers for microtubules, the endoplasmic reticulum and the nucleus. The cells have been grown, fixated and permeabilized before incubation with the antibodies. Confocal microscopy has been used to provide high-resolution, four-color images. The aim of the cell structure section is to highlight the organelle structure of a cell.
The Human Protein Atlas project is funded
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