The Cell Atlas

The Cell Atlas provides high-resolution insights into the expression and spatio-temporal distribution of proteins within human cells. Using a panel of 64 cell lines to represent various cell populations in different organs and tissues of the human body, the mRNA expression of all human genes are characterized by deep RNA-sequencing. The subcellular distribution of each protein is investigated in a subset of cell lines selected based on corresponding gene expression. The protein localization data is derived from antibody-based profiling by immunofluorescence confocal microscopy, and classified into 32 different organelles and fine subcellular structures. The Cell Atlas currently covers 12390 genes (63%) for which there are available antibodies. It offers a database for exploring details of individual genes and proteins of interest, as well as systematically analyzing transcriptomes and proteomes in broader contexts, in order to increase our understanding of human cells.

CELLULAR & ORGANELLE PROTEOMES
The Human Cellular and Organelle Proteome chapters are a collection of interactive pages providing conceptual overviews, compilations and analyses of the data generated within the Cell Atlas from different perspectives. Here you also find basic information about the organelles and subcellular structures classified within the Cell Atlas, together with beautiful example images. The chapters can serve as an educational resource, but also as a starting point for deeper exploration of the fascinating spatiotemporal complexity of transcriptomes and proteomes of human cells. Discover the cell line transcriptome, the organelle proteome, the cell cycle-dependent proteome and the multilocalizing proteome, with the opportunity to take on a holistic view of human cells and its underlying molecular mechanisms.
Cell cycle dependent proteome Multilocalizing proteome Cell line transcriptome Organelle proteome
Nucleus	Nuclear membrane - Nuclear membrane Nucleoli - Nucleoli - Nucleoli fibrillar center Nucleoplasm - Nuclear bodies - Nuclear speckles - Nucleoplasm
Cytoplasm	Actin Filaments - Actin filaments - Focal adhesion sites Centrosome - Centriolar satellite - Centrosome Cytosol - Aggresome - Cytoplasmic bodies - Cytosol - Rods & rings Intermediate Filaments - Intermediate filaments Microtubules - Cleavage furrow - Cytokinetic bridge - Microtubule ends - Microtubules - Midbody - Midbody ring - Mitotic spindle Mitochondria - Mitochondria
Secretory	Endoplasmic Reticulum - Endoplasmic reticulum Golgi Apparatus - Golgi apparatus Plasma Membrane - Cell junctions - Plasma membrane Secreted proteins - Secreted proteins Vesicles - Endosomes - Lipid droplets - Lysosomes - Peroxisomes - Vesicles

A Journey through the Cell
Join us on an inspiring journey through the cell; the basic structural and functional unit of the human body and the home for many of our proteins. This video uses comprehensive animated graphics and microscopy images from the Cell Atlas to give you an overview of the inner universe of a human cell. Travel through a world of organelles and subcellular compartments, and get familiar with their basic structure and unique functions.