A number of educational videos have been produced to show different aspects of the Human Protein Atlas and its content.
Part 1: Alzheimer´s Disease
Part 2: Blood-brain barrier
Dr Evelina Sjöstedt (Karolinska Institutet) is interviewed in this video, where she describes the 3D location of endothelial cells and the star-shaped astrocytes that are components of the Blood-Brain Barrier.
Part 3: Orphan receptor GPR151
This video describes the location of the orphan receptior GPR151 using light sheet immunostaining (GPR151).
Part 4: Insulin
This video shows the location of the protein insulin (INS) in the pancreas. The islets of Langerhans are responsible for producing the protein insulin, regulating the blood sugar levels. When the production of insulin malfunctions, this leads to diabetes.
The video describes the making and the results from the 3D imaging, with an interview of the HPA researcher Dr Cecilia Lindskog, Director of the Tissue Atlas. The location of insulin localized to the beta cells in islets of Langerhans, is visualized in green and the nerves surrounding the blood vessels are seen in red. "By imaging pancreas in 3D, we see that the nerve fibers form a fine network that surrounds the islets of Langerhans. We can also observe that the islets have different sizes, which is not easily seen in normal 2D histological sections. This gives important information for further research on diabetes, in order to find novel treatments for the disease", says Cecilia Lindskog.
Part 5: Introducing the Human Protein Atlas
In this video, the different parts of the HPA portal are described, including the Tissue Atlas, the Subcellular Atlas, the Pathology Atlas, the Blood Atlas, the Brain Atlas and the Metabolic Atlas. All the HPA information about a specific protein-coding gene is collected on the gene-specific Summary Page, including also information about the function of the protein and potential disease involvement.
The Human Protein Atlas data base is a resource encompassing all proteins in the human body, allowing researchers to explore the complex network of these molecules. The presence, or absence, of a particular protein can be studied in all major tissues and organs.
In the video, Mathias Uhlén, Director the HPA is interviewed and 3-D movies generated by light-sheet microscopy from different parts of the human body are shown.
Part 6: Narcolepsy
In this video, the 3D visualization of the neuropeptide orexin (HCRT, Hypocretin neuropeptide precursor) is shown in mouse and human brain, using iDISCO volume imaging technology and light sheet microscopy. The anatomy and function of orexin neurons and the possible mechanism of the sleep disorder narcolepsy are discussed in an interview with neurobiologist Dr. Csaba Adori, Karolinska Institutet, Department of Neuroscience.
Part 7: The nervous heart
In this video the heart is shown in 3D and the protein tyrosine-hydroxylase (TH) has been volume immunostained. Dr. Cecilia Linde, professor in cardiology at Karolinska Institutet, describes the sympathetic nervous system of the heart, which releases noradrenalin.
Introduction to the human cell