Two dipeptidase proteins with different tissue specificity are displayed through multiplex immunohistochemistry (mIHC/IF).

The multiplex antibody-based staining method is able to create illuminated tissue landscapes of chosen cell types or structures where each marker signal can be turned on or off to enhance the visibility of the spatial distribution of a target protein. Dipeptidase 1 and 3 have different tissue specificity and distribution. While dipeptidase 1 (gene: DPEP1) have elevated expression in kidney, intestine and pancreas, dipeptidase 3 (gene: DPEP3) is testis-specific.

In kidney (left side of image), DPEP1 (green) is expressed luminally in proximal tubules, without expression in distal tubules (red), collecting ducts (cyan), glomerulus podocytes (yellow) and endothelia (magenta). In contrast, DPEP3 (green) is expressed in testis (right side of image) in the cytoplasm of developing sperm cells belonging to most of the different stages of the spermatogenesis, including preleptotene (yellow), leptotene (cyan) and pachytene/diplotene (magenta) spermatocytes, as well as early (white) and late (red) spermatids.

For more details about the mIHC/IF technique visit our method page.