Multiplex immunohistochemistry (mIHC/IF) reveals nuclear expression of the protein Shugoshin 1 (gene: SGO1) in pachytene/diplotene spermatocytes - the early cell states of developing sperm cells...Read more

Tripartite motif-containing protein 73 (gene: TRIM73) is a protein whose expression at the protein level was unveiled only recently by a team affiliated with the Human Protein Atlas. Multiplex immunohistochemistry allowed them to confirm its cell-type specific localization in the ovary, thus significantly advancing our understanding of both TRIM73 and the ovary proteome...Read more

How can sperm swim? The outer dense fiber of sperm tails 4 (gene: ODF4) is one of the proteins thought to be important for sperm tail movement that propels the sperm forward. Multiplex immunohistochemistry technique enabled us to examine the expression of this protein during sperm development (spermatogenesis) in more detail than ever before...Read more

The image of the month is a fluorescent antibody-based display of the glomerulus; the main filtrating unit within the kidney generated in the HPA group in Uppsala. The image is a result of a multiplex immunohistochemistry staining, where the podocyte protein podocin (gene: NPHS2) and five other proteins specific to different main structures within the kidney were stained and visualized through fluorescently tagged antibodies...Read more

This month we will look into a protein expressed in testis called lamin B receptor (LBR). The analysis was performed with the new multiplex immunohistochemistry technique, which allowed us to characterize when and where proteins are expressed during spermatogenesis - the development of sperm. More in depth information about the technique can be found here!..Read more