In an article in the Journal of Proteome Research representatives of HUPO's Human Plasma Proteome Project (HPPP), including several HPA affiliated researchers, present the current progress in the study and mapping of the circulating proteome and discuss how updates of proteomics methods, emerging technologies, and investigations of proteoforms and protein networks can impact the understanding of the proteins circulating in blood...Read more

In an Olink-sponsored Labroots webinar on February 4 three key opinion leaders in proteomics will discuss how integrating antibody-based methods with LC- MS ultimately can advance clinical research...Read more

The brain is a high energy consuming tissue that needs a continuous supply of energy substrates from the circulation for optimal neurological function. Astrocytes are important for regulating the relationship between energy consumption and dynamic neuronal activity in the brain and are mainly involved in processes used for efficient transfer of energy substrates to neurons. Changes in astrocyte energy metabolism could therefore potentially disrupt essential neuronal processes, and this cell type has also been shown to undergo structural and biochemical changes in Alzheimer's disease (AD). Here the cell type distribution and cellular levels of the brain enzyme CKB, which is crucial for energy homeostasis and known to have decreased activity in AD, has been investigated to identify changes associated with neurodegenerative processes...Read more

The impact of different trypsins on commonly used bottom-up proteomics workflows were investigated across a range of sample types in a publication in Analytical Chemistry. Besides the type of trypsin, different protease concentrations, sample matrices, instrument acquisition techniques, and protease storage times were investigated...Read more

In a publication in Clinical Proteomics a novel orthogonal strategy based on affinity proteomics and mass spectrometry was used for analytical validation of biomarkers in longitudinally collected serum samples from Duchenne muscular dystrophy (DMD) patients...Read more