Meiosis-associated Boule homolog, RNA binding protein (gene: BOLL) is localized to mid-stage developing sperm in testis with multiplex immunohistochemistry (mIHC/IF).

With the help of the mIHC/IF spermatocytes panel, it is possible to facilitate high resolution spatial localization of proteins that are expressed during the stages of spermatogenesis in which the genetic material of the chromosome pairs are aligned and recombined. Using this panel, Boule homolog, RNA binding protein (BOLL), a protein that has been found to be essential for sperm development, is mainly localized (green) to the cytoplasm of pachytene/diplotene spermatocytes (magenta).

In accordance with the spatial expression pattern, single-cell RNA sequencing data for BOLL shows group enriched expression in different cells of spermatogenesis with the highest expression in mid-stage spermatocytes.

For more details about the mIHC/IF technique visit our method page.